What is the clinical evidence from this study?

Study design: Other. Intervention: Polio recombinant plasmid containing SV40 transcription and replication signals. Primary outcome: Production of infectious poliovirus (plaque-forming units).

January 1, 1984Nucleic Acids ResearchOpen Access

Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals

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Key result

Transfection of COS-1 cells with a polio recombinant plasmid containing SV40 transcription and replication signals yielded greater than 1,600 plaque-forming units per microgram of input DNA.

Population

Cultured primate cells (COS-1 cells) and E. coli

Design

Preclinical

Authors

BSB L SemlerUniversity of California, Irvine ADAndrew J. DornerTakeda (United States)EWEckard WimmerCalifornia Institute of Technology

Discussion

Member takes

Implication

May enable polio vaccine research; leaves open extension to other RNA viruses.

Structured PICO

Population

Cultured primate cells (COS-1 cells) and E. coli

Intervention

Transfection with a polio recombinant plasmid containing SV40 transcription and replication signals

Outcome

Production of infectious poliovirus (plaque-forming units)surrogate

The inclusion of SV40 transcription and replication signals dramatically increases the production of infectious poliovirus from cloned cDNA in primate cells.

Cite This Study

Semler et al. (1984) studied this question. Polio recombinant plasmid containing SV40 transcription and replication signals was evaluated on Production of infectious poliovirus (plaque-forming units). Transfection of COS-1 cells with a polio recombinant plasmid containing SV40 transcription and replication signals yielded greater than 1,600 plaque-forming units per microgram of input DNA.

synapsesocial.com/papers/6a6e82abe5469ee92be0040c https://doi.org/10.1093/nar/12.12.5123

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January 1, 1984Nucleic Acids ResearchOpen Access

Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals

View Full Paper

Ask AI

Bookmark

Key result

Transfection of COS-1 cells with a polio recombinant plasmid containing SV40 transcription and replication signals yielded greater than 1,600 plaque-forming units per microgram of input DNA.

Population

Cultured primate cells (COS-1 cells) and E. coli

Design

Preclinical

Authors

BSB L SemlerUniversity of California, Irvine ADAndrew J. DornerTakeda (United States)EWEckard WimmerCalifornia Institute of Technology

Discussion

Member takes

Implication

May enable polio vaccine research; leaves open extension to other RNA viruses.

Structured PICO

Population

Cultured primate cells (COS-1 cells) and E. coli

Intervention

Transfection with a polio recombinant plasmid containing SV40 transcription and replication signals

Outcome

Production of infectious poliovirus (plaque-forming units)surrogate

The inclusion of SV40 transcription and replication signals dramatically increases the production of infectious poliovirus from cloned cDNA in primate cells.

Cite This Study

synapsesocial.com/papers/6a6e82abe5469ee92be0040c https://doi.org/10.1093/nar/12.12.5123

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

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