November 1, 1998DNA and Cell Biology

Persistence of Heterologous Nucleic Acids after Uptake by Mammalian Cells

View Full Paper

Ask AI

Bookmark

Population

Mammalian cells transfected with plasmids containing the complete genome of poliovirus-1

Design

Preclinical

Follow-up

up to 16 days

Authors

IGIngeborg GraeberRobert Koch Institute JTJENS TISCHER JHJörg C. HeinrichTechnische Universität Dresden

Discussion

Member takes

Implication

Guides timing in cDNA-based viral models; leaves open durability for therapeutic translation.

Structured PICO

Population

Mammalian cells transfected with plasmids containing the complete genome of poliovirus-1

Intervention

Cultivation for up to 16 days under the influence of guanidine or other drugs which inhibit plaque formation, followed by drug removal

Outcome

Infectious activity (plaque-producing activity) of transfected poliovirus cDNAsurrogate

Transfected poliovirus cDNA maintains its infectious capacity in mammalian cells for 8 to 10 days, depending primarily on cDNA stability rather than RNA transcript stability.

Cite This Study

Graeber et al. (1998) studied this question.

synapsesocial.com/papers/6a71666bf44fa9f079df327e https://doi.org/10.1089/dna.1998.17.945

View Full Paper

Ask AI

Bookmark

Persistence of Heterologous Nucleic Acids after Uptake by Mammalian Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Persistence of Heterologous Nucleic Acids after Uptake by Mammalian Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider