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March 1, 1985Journal of VirologyOpen Access

In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA clones of the neurovirulent Mahoney strain and the attenuated Sabin 1 strain

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Population

HeLa S3 cells infected with poliovirus strains

Comparison

Recombinant poliovirus constructed by replacing… vs Parental viruses

Design

Preclinical

Authors

MKMichinori KoharaTokyo Metropolitan Institute of Medical ScienceTOT OmataTokyo Metropolitan Institute of Medical ScienceAKAkihiro KamedaHitotsubashi University

Discussion

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Implication

Hybrid poliovirus traits map attenuation markers; extends mutation-phenotype correlations but leaves open in vivo relevance.

Structured PICO

P
Population
HeLa S3 cells infected with poliovirus strains
I
Intervention
Recombinant poliovirus constructed by replacing PstI fragments (nucleotides 1814-3421) of Sabin 1 cDNA with corresponding fragments from the Mahoney strain cDNA
C
Comparator
Parental viruses (attenuated Sabin 1 strain and neurovirulent Mahoney strain)
O
Outcome
In vitro phenotypic markers (neutralization by monoclonal antibody, bicarbonate concentration dependence, temperature-sensitive multiplication, plaque morphology)surrogate

In vitro recombination of infectious cDNA clones allows for the correlation of specific mutations in an RNA virus genome with its biological characteristics.

Cite This Study

Kohara et al. (1985) studied this question.

synapsesocial.com/papers/6a8525ca38b24859dfd6a044https://doi.org/10.1128/jvi.53.3.786-792.1985
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Also Consider

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  1. 1Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals1984 · 122 citations
  2. 2Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1.1983 · 75 citations
  3. 3Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies1982 · 137 citations
  4. 4Aggregation of Enterovirus Small Plaque Variants and Polioviruses under Low Ionic Strength Conditions1978 · 18 citations
  5. 5Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs.1982 · 250 citations