October 1, 1989Open Access
View Full PaperEffects of iloprost (ZK 36374) on glutathione status during ischaemia and reperfusion of rabbit isolated hearts
Key Result
Pretreatment of rabbit isolated hearts with iloprost at 27 or 270 nM improved myocardial functional recovery, reduced CPK and glutathione release, and attenuated oxidative stress during reperfusion.
Structured PICO
Does iloprost pretreatment improve functional recovery and attenuate oxidative stress in rabbit isolated hearts during ischaemia and reperfusion?
P
Population
Rabbit isolated hearts subjected to 60 min of ischaemia and reperfusion
I
Intervention
Pretreatment with iloprost (stable prostacyclin analogue) at 2.7, 27, or 270 nM
C
Comparator
Untreated rabbit isolated hearts subjected to ischaemia and reperfusion
O
Outcome
Functional recovery of the myocardium and markers of oxidative stress (CPK, GSH, GSSG release, Mn SOD activity)surrogate
Iloprost pretreatment attenuates oxidative stress and improves functional recovery in isolated rabbit hearts subjected to ischaemia-reperfusion injury.
Abstract
Reperfusion of rabbit isolated hearts after 60 min of ischaemia resulted in poor recovery of mechanical function, release of creatine phosphokinase (CPK) and of reduced (GSH) and oxidized (GSSG) glutathione, reduction of mitochondrial superoxide dismutase (Mn SOD) activity and of tissue GSH/GSSG ratio with a shift of cellular thiol redox state toward oxidation, suggesting the occurrence of oxidative stress. 2. Pretreatment of the isolated heart with the stable prostacyclin analogue (iloprost) at 27 or 270 nM, but not at 2.7 nM, improved the functional recovery of the myocardium, reduced CPK, GSH and GSSG release, maintained Mn SOD activity and attenuated the occurrence of oxidative stress. 3. This effect of iloprost cannot be explained by a decreased demand or an enhanced delivery of oxygen during ischaemia or by a direct effect on glutathione peroxidase and reductase activity.