Study on the mechanism of italicDiwu Yanggan Capsule/italic (地五养肝胶囊) -containing serum in regulating macrophage M2 polarization through activating the Nrf2/HO-1 signaling pathway

Key Points

• This research investigates the impact of Diwu Yanggan Capsule (DWYG) serum on macrophage M1/M2 polarization and the underlying mechanisms.
• Induced RAW264.7 cells with LPS and divided into different treatment groups: control, model, and three dosages of DWYG.
• Measured mRNA levels of iNOS, Arg-1, TNF-α, IL-6, IL-10, Nrf2, HO-1, and Keap1 using Real-time RT-PCR.
• Utilized ELISA to evaluate TNF-α, IL-6, and IL-10 protein levels; assessed ROS and GSH/GSSG ratios using flow cytometry and colorimetric assays.
• DWYG serum intervention decreased iNOS, TNF-α, and IL-6 mRNA levels and protein secretion.
• DWYG serum increased Arg-1 and IL-10 mRNA expression, along with IL-10 protein secretion.
• DWYG serum reduced intracellular ROS levels and upregulated Nrf2 and HO-1 protein expression while downregulating Keap1.