Study on the mechanism of Diwu Yanggan Capsule (地五养肝胶囊) -containing serum in regulating macrophage M2 polarization through activating the Nrf2/HO-1 signaling pathway

September 1, 2025Lishizhen medicine and materia medica research

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This research investigates the impact of Diwu Yanggan Capsule (DWYG) serum on macrophage M1/M2 polarization and the underlying mechanisms.
Induced RAW264.7 cells with LPS and divided into different treatment groups: control, model, and three dosages of DWYG.
Measured mRNA levels of iNOS, Arg-1, TNF-α, IL-6, IL-10, Nrf2, HO-1, and Keap1 using Real-time RT-PCR.
Utilized ELISA to evaluate TNF-α, IL-6, and IL-10 protein levels; assessed ROS and GSH/GSSG ratios using flow cytometry and colorimetric assays.
DWYG serum intervention decreased iNOS, TNF-α, and IL-6 mRNA levels and protein secretion.
DWYG serum increased Arg-1 and IL-10 mRNA expression, along with IL-10 protein secretion.
DWYG serum reduced intracellular ROS levels and upregulated Nrf2 and HO-1 protein expression while downregulating Keap1.

Examines Diwu Yanggan Capsule’s effects on macrophage polarization, suggesting anti-inflammatory implications.

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DENG et al. (2025) studied this question.

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