Protective effect of italicSiwu Wuzi Pill/italic（四物五子丸）containing serum on hydrogen peroxide-induced oxidative damage in ARPE-19 cells

Key Points

• The study aims to observe the protective effects of Siwu Wuzi Pill serum against hydrogen peroxide-induced oxidative damage in ARPE-19 cells.
• ARPE-19 cells were induced with H2O2 to form oxidative damage groups including control and treatment groups.
• Treatments involved administering low, medium, and high doses of Siwu Wuzi Pill serum.
• MTT, wound healing assays, and Western blot were used to assess cell viability and protein expression levels.
• H2O2 exposure at 200 μmol/L significantly induced oxidative damage in ARPE-19 cells (P<0.05).
• The serum improved cell proliferation (P<0.05) and vitality, decreasing MDA levels (P<0.001) while increasing CAT activity (P<0.05).
• The intervention significantly lowered the expression of Cat B, Cys C, MMP-2, and TIMP-2 proteins (P<0.05) and their mRNA levels (P<0.01).