What type of study is this?

September 10, 2025

Fixation and immunohistochemical staining of Aiptasia larvae v1

CRChristian CR RenickeJohns Hopkins University

Puntos clave

High-quality images of Aiptasia larvae can be captured using fluorescence microscopy techniques.
Effective fixation preserves key structures such as microtubules and filamentous actin in these organisms.
This method allows for assessment of Aiptasia larvae’s morphology, including size and gastric cavity.
Using a confocal laser-scanning microscope enhances image quality for detailed analysis.

Resumen

This protocol is for fixation of Aiptasia (Exaiptasia diaphana) larvae and subsequent staining of microtubules, filamentous actin and nuclei. The resulting samples can be imaged under a widefield fluorescence microscope with sufficient image quality to assess basic morphological features such as size, gastric cavity and pharynx, cilia and apical tuft. For highest quality images, a confocal laser-scanning microscope can be used.

Preguntar a la IA

Me gusta

Guardar

Ver artículo completo

Cite This Study

Christian CR Renicke (Sat,) studied this question.

synapsesocial.com/papers/68c189d99b7b07f3a0613715 https://doi.org/https://doi.org/10.17504/protocols.io.14egnyqpqv5d/v1

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

Preguntar a la IA

Me gusta

Guardar

Ver artículo completo