HClO activated endoplasmic reticulum fluorescent probe for visualization of ferroptosis-mediated acute alcoholic gastric and liver injury

Key Points

• The aim is to develop and validate a fluorescent probe for detecting hypochlorous acid in the endoplasmic reticulum during ferroptosis-related injuries.
• Developed a near-infrared fluorescent probe (ER-HClO) for detecting HClO.
• Evaluated the probe's effectiveness in visualizing HClO levels.
• Applied the probe in models of acute alcoholic gastric and liver injury.
• Demonstrated increased levels of HClO in the endoplasmic reticulum during ferroptosis.
• Visual evidence indicates a significant association between HClO and acute injury.
• The probe successfully highlights ferroptosis processes in real-time.