What question did this study set out to answer?

The aim is to enhance the quality of cryopreserved chicken sperm through optimal dilution rates and DMSO concentrations.

March 13, 2026Open Access

Enhancing Native Chicken Sperm Cryopreservation Efficiency via Pellet Method Optimization

Key Points

The aim is to enhance the quality of cryopreserved chicken sperm through optimal dilution rates and DMSO concentrations.
Collected semen from KUB roosters via abdominal massage.
Tested dilution rates of 1 × 10^9 and 1.5 × 10^9 cells/mL.
Evaluated two DMSO concentrations (6% and 9%).
Assessed equilibration times of 1 minute and 30 minutes at 4 °C.
Measured post-thaw sperm motility, viability, and recovery rates.
Optimal conditions were 1 × 10^9 cells/mL dilution, 6% DMSO, and 1-minute equilibration.
Post-thaw sperm motility varied significantly based on equilibration time and DMSO concentration.
Fertility and hatchability rates for frozen semen were similar to fresh semen.

Abstract

ABSTRACT Cryopreservation of chicken sperm remains challenging due to reduced post-thaw semen quality and artificial insemination (AI) success. This study aimed to optimize dilution rate, dimethyl sulfoxide (DMSO) concentration, and equilibration time to preserve the quality of Kampung Unggul Badan Litbang (KUB) chicken semen. Semen from ten KUB roosters was collected twice weekly via abdominal massage, and diluted to 1 × 109 or 1.5 × 109 cells/mL. Two DMSO concentrations (6% and 9%) and two equilibration durations (1 min and 30 min at 4 °C) were tested. Post-thaw sperm motility differed significantly (p0.05). These findings support the development of improved cryopreservation protocols for KUB chicken semen and may offer practical value for poultry breeding programs in tropical regions.

Enhancing Native Chicken Sperm Cryopreservation Efficiency via Pellet Method Optimization

Key Points

Abstract

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