Optimizes sperm cryopreservation techniques in KUB chickens, suggesting benefits for poultry breeding.
Cryopreservation of chicken sperm remains challenging due to reduced post-thaw semen quality and artificial insemination (AI) success. This study aimed to optimize dilution rate, dimethyl sulfoxide (DMSO) concentration, and equilibration time to preserve the quality of Kampung Unggul Badan Litbang (KUB) chicken semen. Semen from ten KUB roosters was collected twice weekly via abdominal massage, and diluted to 1 × 109 or 1.5 × 109 cells/mL. Two DMSO concentrations (6% and 9%) and two equilibration durations (1 min and 30 min at 4 °C) were tested. Post-thaw sperm motility differed significantly (p<0.05) across equilibration times and DMSO concentrations. The combination of 1×109 cells/mL dilution, 6% DMSO, and 1-minute equilibration yielded optimal motility, viability, and recovery rates. Fertility and hatchability of frozen semen were comparable to fresh semen (p>0.05). These findings support the development of improved cryopreservation protocols for KUB chicken semen and may offer practical value for poultry breeding programs in tropical regions.
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Kostaman et al. (2025) studied this question.
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