SK Kedu Semen Cryopreservation in Beltsville Poultry Semen Extender and Lactated Ringer’s-Egg Yolk Extender Using Dimethyl Sulfoxide

Objective: This study was conducted to evaluate frozen semen quality of SK kedu chicken after cryopreservation in Beltsville Poultry Semen Extender (BPSE) and lactated ringer's-egg yolk (LR-EY) with 8, 10 and 12% concentrations of dimethyl sulfoxide (DMSO).Methodology: Semen was collected from 5 SK kedu roosters using cloaca massage technique twice a week.Semen was evaluated macro and microscopycally then polled and divided into 6 tubes of treatments.Three tubes were diluted in BPSED 8 , BPSED 10 , BPSED 12 and three other tubes were diluted in LR-EYD 8 , LR-EYD 10 , LR-EYD 12 .Semen of each treatments loaded into 0.25 mL straw, equilibrated at 5EC for 2 h.Freeze above nitrogen vapor and stored in container of liquid nitrogen at -196EC, then semen thawed in a water bath at 37EC for 30 sec.Data was analyzed using Statistical Analysis System (SAS).Results: Result showed that post thawing sperm motility in LR-EYD 10 (40.83±1.67%)was higher (p<0.05)than other treatments.The sperm recovery rate in LR-EYD 10 (46.71±1.97%)was higher than other treatments (p<0.05).Sperm viability was not differ post dilution, post equilibration and post thawing in all treatments.Conclusion: This study showed that LR-EY with 10% DMSO (LR-EYD 10 ) was better to maintain sperm motility and sperm recovery rate of SK kedu frozen semen.