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Autoregulation of three yeast ribosomal protein genes by splicing inhibition | Synapse

April 1, 2026Open Access

Autoregulation of three yeast ribosomal protein genes by splicing inhibition

Key Points

To investigate how splicing inhibition affects the autoregulation of specific yeast ribosomal protein genes.
Inserted introns of ribosomal protein genes into chromosomal GFP
Induced expression of cognate proteins
Observed repression of GFP as a measure of regulation
RPL18B and RPS22B require a conserved secondary structure in the intron for regulation
RPL28 does not rely on the conserved structure for its regulation
Splicing inhibition leads to decreased expression of ribosomal protein-related GFP

Abstract

Abstract Yeast ribosomal protein genes RPL18B, RPL28 and RPS22B are autoregulated by inhibition of splicing. This is demonstrated by inserting their introns into a chromosomal copy of GFP and observing repression upon induction of the cognate protein. In RPL18B and RPS22B, a predicted conserved secondary structure within the intron is required for regulation, while in RPL28 it is not.

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Cite This Study

Granas et al. (Mon,) studied this question.

synapsesocial.com/papers/69cd7b155652765b073a8cf2 https://doi.org/https://doi.org/10.1093/g3journal/jkag083