Inhibition of Bilirubin Generation Exacerbates Oxidative Stress and Liver Injury in Early‐to‐Intermediate Obstructive Cholestasis

Key Points

• To investigate the impact of bilirubin inhibition on oxidative stress and liver injury in obstructive cholestasis.
• Assessment of HO1 inhibition effects on bilirubin levels.
• Evaluation of oxidative stress markers in liver tissues.
• Analysis of liver injury indicators in an obstructive cholestasis model.
• Inhibition of bilirubin led to increased markers of oxidative stress.
• Loss of bilirubin exacerbated liver damage during obstructive cholestasis.
• Bilirubin accumulation was found to be protective against bile salt toxicity.