Evaluation of differentiation of the umbilical cord blood-derived mesenchymal stem cells into male germ-line cells in two-dimensional and three-dimensional scaffolds containing naringin.

Key Points

• The research aims to evaluate the differentiation potential of umbilical cord blood-derived mesenchymal stem cells into male germ-like cells using different scaffold designs.
• Utilized 2D and 3D scaffolds containing naringin to promote cell differentiation.
• Assessed cell differentiation efficacy in 3D PLLA/GNF/Nar scaffolds.
• Evaluated microenvironment conditions for optimal cellular development.
• 3D PLLA/GNF/Nar scaffold significantly enhanced differentiation into male germ-like cells.
• The favorable microenvironment in 3D scaffolds suggests improved outcomes compared to 2D scaffolds.
• Findings indicate potential applications in reproductive tissue engineering.