Cryoinfarction in mice resulted in decreased LV fractional shortening comparable to coronary ligation at 4 weeks, but with delayed progressive adverse remodeling occurring between 4 and 8 weeks.
Does cryoinfarction provide a more reproducible model of myocardial infarction compared to coronary artery ligation in mice?
A novel murine cryoinfarction model provides highly reproducible anterior myocardial infarcts with progressive adverse remodeling, serving as a clinically representative alternative to coronary ligation.
Mouse myocardial infarction (MI) models are frequently used research tools. The most commonly applied model is coronary artery ligation. However, coronary ligation often gives rise to apical aneurysmatic infarcts of variable size. Other infarct models include cryoinfarction, which produces reproducible infarcts of the anterior wall. Thus far, this model has not been extensively described in mice. Therefore, we developed a murine cryoinfarction model and compared it with coronary ligation. Studies were performed under isoflurane anesthesia with a follow-up of 4 and 8 wk. Cryoinfarction was induced using a 2- or 3-mm cryoprobe. Two-dimensional guided M-mode echocardiography was used to assess fractional shortening and left ventricular (LV) dimensions at baseline and end point. At end point, hemodynamics were assessed using a 1.4-Fr Millar catheter. Pressure-diameter relations were constructed by combining echocardiography and hemodynamic data. Histological and morphometric analyses of infarct and remote areas were performed. At 4 wk, 3-mm cryoinfarction resulted in decreased LV fractional shortening as well as decreased global LV contractility and relaxation, which was comparable with coronary ligation. No adverse remodeling was observed at this time point, in contrast with the ligation model. However, progressive LV remodeling occured between 4 and 8 wk after cryoinfarction with a further decline in hemodynamic parameters and LV pump function. Histologically, cryoinfarction resulted in highly reproducible, transmural, cone-shaped infarcts with reperfusion at the macrovascular level. These results indicate that the cryoinfarction model represents the anterior myocardial infarct with modest adverse remodeling and may thus be representative for infarcts encountered in clinical practice.
Bos et al. (Fri,) conducted a other in Myocardial infarction (mouse model). Cryoinfarction vs. Coronary artery ligation was evaluated on LV fractional shortening, hemodynamics, and remodeling. Cryoinfarction in mice resulted in decreased LV fractional shortening comparable to coronary ligation at 4 weeks, but with delayed progressive adverse remodeling occurring between 4 and 8 weeks.
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