What question did this study set out to answer?

To determine the specific downstream cellular responses directly regulated by phosphatidylinositol-3 kinase using a constitutively active mutant enzyme.

April 7, 1995Science

Ras-Dependent Induction of Cellular Responses by Constitutively Active Phosphatidylinositol-3 Kinase

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Authors

QHQianjin Hu AKAnke Klippel AMAnthony J. Muslin

Discussion

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Overview

Experimental study demonstrates that active phosphatidylinositol-3 kinase drives gene transcription and oocyte maturation via Ras activation, highlighting PI-3 kinase as an upstream regulator of Ras.

Key Points

To determine the specific downstream cellular responses directly regulated by phosphatidylinositol-3 kinase using a constitutively active mutant enzyme.
Engineered a constitutively active mutant of the PI-3 kinase catalytic subunit (p110) that operates independently of growth factor stimulation.
Expressed p110 in NIH 3T3 cells and Xenopus laevis oocytes with or without dominant-negative Ras to evaluate signaling and phenotypic outcomes.
Expression of active p110 in NIH 3T3 cells induced transcription from the fos promoter, an effect specifically blocked by co-expression of dominant-negative Ras.
In Xenopus laevis oocytes, p110 increased GTP-bound active Ras, stimulated the downstream effector Raf-1, and induced Ras-dependent oocyte maturation.

Cite This Study

Hu et al. (1995) studied this question.

synapsesocial.com/papers/6a1d16217f448865515dc05a https://doi.org/10.1126/science.7701328

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April 7, 1995Science

Ras-Dependent Induction of Cellular Responses by Constitutively Active Phosphatidylinositol-3 Kinase

View Full Paper

Ask AI

Bookmark

Authors

QHQianjin Hu AKAnke Klippel AMAnthony J. Muslin

Discussion

Member takes

Overview

Experimental study demonstrates that active phosphatidylinositol-3 kinase drives gene transcription and oocyte maturation via Ras activation, highlighting PI-3 kinase as an upstream regulator of Ras.

Key Points

To determine the specific downstream cellular responses directly regulated by phosphatidylinositol-3 kinase using a constitutively active mutant enzyme.
Engineered a constitutively active mutant of the PI-3 kinase catalytic subunit (p110) that operates independently of growth factor stimulation.
Expressed p110 in NIH 3T3 cells and Xenopus laevis oocytes with or without dominant-negative Ras to evaluate signaling and phenotypic outcomes.
Expression of active p110 in NIH 3T3 cells induced transcription from the fos promoter, an effect specifically blocked by co-expression of dominant-negative Ras.
In Xenopus laevis oocytes, p110 increased GTP-bound active Ras, stimulated the downstream effector Raf-1, and induced Ras-dependent oocyte maturation.

Cite This Study

Hu et al. (1995) studied this question.

synapsesocial.com/papers/6a1d16217f448865515dc05a https://doi.org/10.1126/science.7701328

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