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May 1, 1995The Journal of Cell Biology273 citationsOpen Access

Immunolocalization of Dihydropyridine, Triadin, and Ryanodine Receptors in Rabbit Heart

Immunolocalization of sarcolemmal dihydropyridine receptor and sarcoplasmic reticular triadin and ryanodine receptor in rabbit ventricle and atrium.

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Population

Adult rabbit ventricle and atrium cells

Design

Preclinical

Key result

In adult rabbit ventricle and atrium, DHPR codistributes with triadin and RyR, suggesting some SR Ca2+ release channels in the atrium may be activated without close association with DHPR.

Authors

SCSöderberg CarlKFK FelixACAnthony H. Caswell

Discussion

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Overview

Hypothesis-generating for distinct atrial Ca2+ release; requires human validation before any clinical relevance.

Key Points

  • Determine the subcellular distribution and structural organization of sarcolemmal dihydropyridine receptors, triadin, and ryanodine receptors in adult rabbit ventricular and atrial myocytes.
  • Performed double-labeling immunofluorescence and laser scanning confocal microscopy to assess the spatial codistribution of DHPR, triadin, and RyR in adult rabbit ventricle and atrium.
  • Used electron microscopy to evaluate cardiac ultrastructure in interior cytoplasmic regions displaying specific calcium channel immunoreactivity.
  • Ventricular myocytes showed nearly complete spatial overlap of DHPR, triadin, and RyR arranged in transverse punctate bands at ~2-micron intervals along dyadic couplings.
  • Atrial myocytes showed peripheral colocalization of all three proteins at the sarcolemma, whereas interior transverse bands spaced at ~2-micron intervals contained only triadin and RyR localized to extended junctional sarcoplasmic reticulum.

Structured PICO

P
Population
Adult rabbit ventricle and atrium cells
I
Intervention
Double labeling immunofluorescence and laser scanning confocal microscopy
O
Outcome
Subcellular distribution and co-localization of sarcolemmal dihydropyridine receptor (DHPR), sarcoplasmic reticular triadin, and ryanodine receptor (RyR)surrogate

This study maps the subcellular localization of key calcium handling proteins in rabbit cardiac tissue, revealing structural differences between ventricular and atrial excitation-contraction coupling.

Cite This Study

Carl et al. (1995) studied this question. In adult rabbit ventricle and atrium, DHPR codistributes with triadin and RyR, suggesting some SR Ca2+ release channels in the atrium may be activated without close association with DHPR.

synapsesocial.com/papers/6a15b0b915658026c082ab15https://doi.org/10.1083/jcb.129.3.673
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Excitation‐contraction coupling in the heart: the state of the question 11992 · 85 citations
  2. 2Reconstitution of the voltage-sensitive calcium channel purified from skeletal muscle transverse tubules1986 · 157 citations
  3. 3The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle.1993 · 134 citations
  4. 4Structural and Functional Characterization of the Purified Cardiac Ryanodine Receptor-Ca2+ Release Channel Complex1989 · 208 citations
  5. 5Ca2+‐ATPase distributes differently in cardiac sarcolemma than dihydropyridine receptor α1 subunit and Na+/Ca2+ exchanger1994 · 14 citations