The Ability of Basic and Glycosylated Proline-Rich Proteins to Protect Oral Enzymes from Polyphenol Inhibition

Alena Proskura; Mariam Muradova; Nicolas Poirier; Victoria Ilina; Jeanne Chaloyard; Valentin Boichot; Mathieu Schwartz; Denis Baranenko; Людмила Надточий; Francis Canon; Fabrice Neiers; Jean‐Marie Heydel

doi:10.1021/acs.jafc.5c00821

August 6, 2025Journal of Agricultural and Food Chemistry

The Ability of Basic and Glycosylated Proline-Rich Proteins to Protect Oral Enzymes from Polyphenol Inhibition

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Key Points

Glycosylated proline-rich proteins show substantial protection against polyphenols for oral enzymes.
Basic proline-rich proteins provide limited enzyme protection at specific polyphenol ratios.
Recombinant proline-rich proteins were tested to assess their protective effects against tannins and flavan-3-ols.
This research advances understanding of how saliva proteins can mitigate the impact of antinutritional polyphenols.],
simple_explanation':'Proline-rich proteins (PRPs) help protect enzymes in our mouth from harmful compounds called polyphenols, like tannins. The study found that glycosylated PRPs are better at this protection than basic PRPs. This matters because protecting oral enzymes can help in breaking down food properly and improving taste. 🥗'} . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , . . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , } . , - } . , } . . , } . , } . , } . . , - } . - }} . } . , - } . , } . } . } . . } . , } . , } . , } - } . , - } . , } . , } . , } . , } . . , } . . . , - } . - } . - . } . . . } . . } . . . } . . } . , - } . . . . . } - } } . - } . } . - }} . . . . } . . . - - } . . . . - - }} - . } . . } . - . } . . . } . . . - } . . - - } - } . . . } . . . - . } - } . - } . . - - } , . } . , } . . . , - } . - } , } . . - - . - - . , } . - . - } . } . . . } . - - . - . } , - } . - } . } - } , . . } - . } . . . . , - . . - } . . , } . - } - . - - . - - } . - . - } , } . . . . , - . . - . - . } - } - . - } . . , - . - } . , } . - - } , . . - . , - } . - - } . , - . - - } . , - ] . } , - } . } . } . . . . } . . } . } . - } - - . - . }} . . . , . } - - . . }} - - . . . - . - . - } , } . } - - } . . } , - ... . . . - - , - . - } , } . . - } . . , , } - . . . , . - - } . } - - , - . - . , . . . - , . . - } . - } - - } , - . . - . . } - - } - . } .
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Implication

Research demonstrates glycosylated proline-rich proteins significantly protect β-glucosidase and glutathione transferase from polyphenol inhibition during oral processing.

Abstract

Tannins and flavan-3-ols can exhibit astringency and potential antinutritional effects by binding, aggregating, and precipitating proteins, including digestive and oral enzymes. During oral food processing, these polyphenolic compounds may inhibit enzymes, such as β-glucosidase and glutathione transferase (GSTP1), which help generate taste-active molecules or transform food components for elimination. Saliva contains proline-rich proteins (PRPs) with high affinity for tannins and flavan-3-ols, potentially protecting oral enzymes from inhibition. This study investigates the ability of two PRP classes─basic (bPRPs) and glycosylated (gPRPs)─to protect β-glucosidase and GSTP1 from polyphenols such as tannins and flavan-3-ols. Recombinant PRPs were produced, purified, and tested for their protective effects on the enzyme activity in the presence of epigallocatechin gallate (EGCG) and tannic acid. Limited protection by bPRP was observed for GSTP1, notably at a PRP-to-polyphenol ratio of 5:1 with either EGCG or tannic acid and at 10:1 only with tannic acid. In contrast, gPRP significantly protected both β-glucosidase and GSTP1 under various conditions involving EGCG and tannic acid.

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Cite This Study

Proskura et al. (2025) studied this question.

synapsesocial.com/papers/689522129f4f1c896c42995a https://doi.org/10.1021/acs.jafc.5c00821

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The Ability of Basic and Glycosylated Proline-Rich Proteins to Protect Oral Enzymes from Polyphenol Inhibition

Key Points

Implication

Abstract

Discussion

Cite This Study

The Ability of Basic and Glycosylated Proline-Rich Proteins to Protect Oral Enzymes from Polyphenol Inhibition

Key Points

Implication

Abstract

Discussion

Cite This Study

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