July 15, 2025Open Access

A chemically defined medium to support the growth of food-relevant Bacillus species

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MAIN FINDING: The Pafoba medium supports growth of thirteen Bacillus strains relevant to food, including pathogenic and food-grade species.
KEY EVIDENCE: Ten Bacillus strains showed equal or higher growth on Pafoba compared to rich media, suggesting its effectiveness.
APPROACH: Experiments included two pathogenic Bacillus cereus strains and eleven Bacillus subtilis strains, analyzing their growth on Pafoba.
SIGNIFICANCE: Pafoba medium facilitates controlled studies on Bacillus spp., aiding food safety and processing research.

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Development of Pafoba medium enhances growth of Bacillus cereus and Bacillus subtilis, indicating improved experimental consistency.

Canoy et al. (2025) studied this question.

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