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This research investigates how Bacillus spp. can inhibit the growth of harmful bacteria in livestock.

May 24, 2026JDS CommunicationsOpen Access

Co-incubation of Bacillus spp. alleviated the growth and gas production of potentially harmful bacteria in different in vitro methodologies

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This research investigates how Bacillus spp. can inhibit the growth of harmful bacteria in livestock.
Conducted in vitro experiments using Bacillus licheniformis 809 or Bacillus subtilis 810 and Clostridium perfringens type A.
Evaluated gas production and bacterial counts using the Ankom RF system bottle.
Performed agar-diffusion assays to assess the inhibitory effects on various bacterial strains.
Co-incubation of Bacillus strains reduced gas production from C. perfringens by 63% for B. licheniformis and 69% for B. subtilis.
Inhibition zones for C. perfringens ranged from 10.5 mm (type C) to 15.5 mm (types A and E).
Clearance zones of 11.5 mm and 8.7 mm were observed for T. pyogenes and F. necrophorum, respectively.

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Randomized trials evaluate Bacillus spp. effects on harmful bacteria growth in livestock, suggesting potential benefits.

Capern et al. (2026) studied this question.

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