July 16, 2025

Structural and enzymatic divergence between human MDH isoforms underlies their specialized regulatory roles in metabolism

View Full Paper

Ask AI

Bookmark

Authors

Discussion

Comparative analysis shows distinct regulatory roles of human MDH isoforms hMDH1 and hMDH2 in metabolism, suggesting unique metabolic adaptations.

hMDH2 demonstrates inhibition by metabolites like α-ketoglutarate and citrate, reflecting its regulatory function in mitochondrial metabolism.
In contrast, hMDH1 shows minimal responses to these metabolites, emphasizing its stable role in cytosolic metabolism.
Structural and model analyses reveal differences in flexibility and electrostatics between the two isoforms, impacting their activities.
These findings indicate that MDH isoform regulation hinges on local structural variations rather than large conformational changes.

Berndsen et al. (2025) studied this question.

View Full Paper

Ask AI

Bookmark

Comparative analysis shows distinct regulatory roles of human MDH isoforms hMDH1 and hMDH2 in metabolism, suggesting unique metabolic adaptations.