CDK1-dependent N-terminal NuMA phosphorylation promotes dynein-dynactin-NuMA assembly for accurate chromosome segregation

The microtubule-based motor dynein and its cofactor dynactin fulfil essential functions throughout the cell cycle, including organelle transport and mitotic spindle assembly. To achieve these diverse functions, dynein-dynactin associates with different activating adaptors. Nuclear Mitotic Apparatus (NuMA) is a mitosis-specific adaptor that connects dynein-dynactin with microtubules to focus mitotic spindle poles. NuMA′s C-terminal microtubule-binding activity is promoted by mitotic phosphorylation, but regulation of NuMA′s N-terminal interaction with dynein-dynactin remains unclear. Here, we combine a membrane-tethering assay, quantitative proteomics, and live functional analyses in human cells to show that the interaction between NuMA′s N-terminus and dynein-dynactin is cell cycle-regulated and driven by mitotic phosphorylation. We identify highly conserved CDK1 consensus sites proximal to NuMA′s dynein heavy chain-binding site, which are phosphorylated by CDK1-Cyclin B1 in cells and in vitro. This CDK1-dependent phosphorylation, together with NuMA′s Spindly-like motif, is crucial for stable dynein-dynactin-NuMA (DDN) complex formation. Replacement of endogenous NuMA with phosphorylation-deficient NuMA mutants leads to aberrant dynein distribution on mitotic spindles, resulting in chromosome mis-segregation and micronucleus formation. Together, our results highlight CDK1-dependent N-terminal NuMA phosphorylation as a crucial mitotic switch that constitutes a regulatable core of multivalent interactions with dynein-dynactin to assemble stable DDN complexes for accurate chromosome segregation.