This protocol demonstrates 3D analysis of fluorescent labeling in microscopy, suggesting a new approach for colocalization studies.
This protocol introduces Bioloc3D, a fully open-source tool designed to quantify fluorescent labeling across entire stacks and automate statistical analysis. It offers a solution to the lack of tools capable of colocalization quantification between multiple identified elements in 3D. Expected results includes graphical representations of the results through images and estimation plots.
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Dhellemmes et al. (2025) studied this question.
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