Abstract Background: Colon and gastric cancers are among the most prevalent gastrointestinal malignancies, often exhibiting poor prognosis due to resistance and recurrence. Polo-like kinase 1 (PLK1), a key regulator of mitosis, is frequently overexpressed in these cancers. BI-2536, a selective PLK1 inhibitor, has shown promising anticancer activity. β-glucan, a natural immunomodulator, has also demonstrated anticancer potential. Objective: This study aimed to evaluate the antiproliferative, apoptotic, and cell cycle effects of BI-2536 alone and in combination with β-glucan on HT-29 colon and AGS gastric cancer cell lines. Methods: Cell viability was assessed by XTT assay. Apoptosis and cell cycle profiles were evaluated by flow cytometry. The combination index (CI) was calculated using the Chou–Talalay method via CompuSyn software. Results: BI-2536 significantly inhibited proliferation and induced G2/M arrest and apoptosis in both cell lines. β-glucan showed moderate cytotoxicity and enhanced BI-2536’s effects in combination. Synergistic antiproliferative activity was observed at lower drug concentrations (CI Conclusion: BI-2536 in combination with β-glucan exhibits synergistic anticancer effects in vitro, suggesting a promising strategy for treating colon and gastric cancers. Further in vivo studies are warranted.
TAKCI et al. (Thu,) studied this question.
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