Technical treatments of Antioxidant Test with DPPH Method affecting IC50 value

Antioxidant tests have been applied in various tests of foods and beverages in various forms, even in nanoscale. Information on antioxidant activity is important as an indicator that handles free radicals. So far, many antioxidant tests have used DPPH reagent, commonly known as the DPPH method. By referring to SNI/AOAC, its application is interesting to study. Anticipating error treatments in this antioxidant test is important for obtaining valid results and reducing errors resulting from the technical treatments. Many studies presented various uncorrected IC50 values because of various treatments. From preparation to analysis of results, there is a potential for error that is important to note. Errors can be due to tester errors, tool errors, material errors, and room condition errors. These error results cause the IC50 value to be bad/invalid because the linear regression curve graph is poor, and incorrect. This study examined all errors affected the IC50 values studies.