Antioxidant testing is widely needed in health aspects. Knowledge of antioxidant values can help in the selection and development of food products. The DPPH method is widely used because of its ease and efficiency. For plant samples, initial treatment is needed to ensure the accuracy of the solution concentration. Many plant samples must be extracted first. This process is time consuming and has mass validity issues. This study aims to: to evaluate a DPPH antioxidant test method without extraction that is efficient, effective, practical, and environmentally friendly, (2) to analyze the validity of the developed method. The result revealed that the IC50 value was obtained about 1028.85 ppm. The linearity and precision were 0.98 and 0.02% respectively. This method proved that without extraction stage, the IC50 can be determined.
Asrori et al. (2025) studied this question.