Comparative Analysis of Methanol and Ethanol Extract of Flammulina velutipes on Primary Hippocampal Culture: From Neurodevelopment to Neuroprotection

Flammulina velutipes is a well-known edible mushroom which is consumed for its nutri-tional value. F. velutipes is also known as enoki mushroom, a good source of antioxidants, fiber, polysaccharides, mycosterol and minerals that make it a nutritious functional food. In this present study we utilized methanol and ethanol extract of F. velutipes mushroom on primary neuronal cultured cell and made a comparative study on neuronal develop-ment, cytoarchitecture, neuronal viability and maturation. We also characterized the methanolic and ethanolic extracts of mushroom with GC-MS profiling and detected the active metabolites present in the extracts. The comparative study showed methanolic ex-tract of the mushroom has more promising impact on neuronal development and neuri-togenesis. Furthermore, we evaluated the mechanistic insight and found that the impact of the mushroom is dependent on NTRK receptor. Additionally, we validated the activa-tion of NTRK receptor by in-silico molecular docking study.