Supplementary Figure S3 from Highly Sensitive DNA Testing of Fusobacterium nucleatum in Colorectal Tumors

Meredith A.J. Hullar; Orsalem J. Kahsai; Courtney Hill; Lisa Levy; Rachel C. Malen; Keith R. Curtis; Hamza Ammar; Arthur Sillah; Adriana M. Reedy; Johanna W. Lampe; Shuji Ogino; John D. Potter; Polly A. Newcomb; Amanda I. Phipps

doi:10.1158/1055-9965.29762043

August 1, 2025

Supplementary Figure S3 from Highly Sensitive DNA Testing of Fusobacterium nucleatum in Colorectal Tumors

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Key Points

MAIN FINDING: The study identifies the limit of detection and quantitation for fusobacterium nucleatum in colorectal tumors.
KEY EVIDENCE: Serial dilution of nusG gene copies demonstrates sensitivity in detecting fusobacterium nucleatum.
APPROACH: The method involves adding gene copies to extracted human genomic DNA from FFPE tissues and using ddPCR.
SIGNIFICANCE: This research enhances the ability to detect fusobacterium nucleatum, potentially improving colorectal cancer diagnostics.

Implication

Testing reveals limit of detection and quantitation for fusobacterium nucleatum in human genomic DNA, indicating ddPCR’s effectiveness.

Abstract

Supplementary Figure S3: Serial dilution of nusG gene copies added to human genomic DNA extracted from FFPE human tissue to determine the limit of detection (LOD) and limit of quantitation (LOQ) of nusG using ddPCR.

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Cite This Study

Hullar et al. (2025) studied this question.

synapsesocial.com/papers/689a0c72e6551bb0af8d0100 https://doi.org/10.1158/1055-9965.29762043

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Supplementary Figure S3 from Highly Sensitive DNA Testing of Fusobacterium nucleatum in Colorectal Tumors

Key Points

Implication

Abstract

Discussion

Cite This Study

Supplementary Figure S3 from Highly Sensitive DNA Testing of Fusobacterium nucleatum in Colorectal Tumors

Key Points

Implication

Abstract

Discussion

Cite This Study

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