Supplementary Figure S2 from Comprehensive Single-Cell RNA Atlas of Human Laryngeal Normal, Preneoplastic, and Tumorigenic States

Supplementary Figure S2. (A) The tSNE plots results of individual samples within the VCP and VCL groups. (B) The tSNE plots results of individual samples within the LSCC and LSCCP groups. (C) t-SNE plots showing the main distribution in each of the seven cell types identified in the four types of tissue samples analyzed in this study. (D) Principal Component Analysis (PCA) of Sample Groups. Illustrates the clustering of LSCC, LSCCP, VCL, and VCP samples based on principal components. (E) Comparison of Euclidean Distances Within and Between Groups. Violin plot showing the Euclidean distance distribution between-group and within-group, with statistical significance (Wilcoxon test, p = 7.8e-07). (F) Gene statistics and unique molecular identifiers (UMIs) of all cell types in different types of samples in this study. (G) Incoming and outgoing cell-cell communication intensities among the seven major cell types across the four tissue groups. (H) The stacked bar plots of cell type and subcluster proportions in each sample. a. All cell type; b. epithelial cell subclusters; c. fibroblast subclusters; d. endothelial cell subclusters. (I) The stacked bar plots of cell type and subcluster proportions in each sample. a. T cell subclusters; b. T cell type; c. B cell subclusters; d. B cell type; e. myeloid cell subclusters; f. myeloid cell type.