August 4, 2025

Introducing Quantitative Assessment of Michaelis Constant (Km) Accuracy

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Authors

TWTong WangTulane University PDParmeetpal DhillonYork University SKSvetlana M. KrylovaYork University

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Implication

This framework demonstrates accurate Km values in enzyme kinetics, indicating better decision-making in metabolic-flux models and inhibitor screenings.

Key Points

MAIN FINDING: The binding-isotherm framework enhances the accuracy of the Michaelis constant measurements.
KEY EVIDENCE: Standard software often underestimates true Km uncertainty, while the ACI provides reliable confidence intervals.
APPROACH: The method adapts traditional velocity-versus-substrate fits to enable accuracy assessment without extra experiments.
SIGNIFICANCE: This improves enzyme variant selection and metabolic modeling, minimizing inefficiencies in bioprocesses.

Cite This Study

Wang et al. (2025) studied this question.

synapsesocial.com/papers/689a0f86e6551bb0af8d0ac1 https://doi.org/10.26434/chemrxiv-2025-tkrnx

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Implication

This framework demonstrates accurate Km values in enzyme kinetics, indicating better decision-making in metabolic-flux models and inhibitor screenings.

Introducing Quantitative Assessment of Michaelis Constant (Km) Accuracy

Discussion

Member takes

Implication

Key Points

Cite This Study

Introducing Quantitative Assessment of Michaelis Constant (Km) Accuracy

Discussion

Member takes

Implication

Key Points

Cite This Study

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Also Consider