TapeStation 4150: DNA Electrophoresis v1

The two kits we will generally be using with the TapeStation 4150 are the D1000 kit and the Genomic DNA kit. The D1000 kit has a sizing range of 35 to 1000 bp and a quantitative range of 0.1-50 ng/µL. The Genomic DNA kit has a sizing range of 200 to >60000 bp, a quantitative range of 10-100 ng/µL, and a DIN (DNA Integrity Number) functional range of 5-300 ng/µL. The protocol between these two kits is very similar, with only the volume of buffer needed for each sample changing (3µL for the D1000 and 10µL for the genomic kit). The D1000 kit should be used for visualizing amplified products of a specific size range (NGS libraries, sexing, mitochondrial sequencing, etc.) and the genomic kit should be used post extraction for assessing the DNA quality of dead whale or known poor quality samples.