A novel method for the quantification of Cocamidopropyl betaine through High Performance Liquid Chromatography coupled with a Refractive Index Detector

Abstract Cocamidopropyl betaine (CAPB) is a moderate zwitterionic (amphoteric) synthetic surfactants commonly used in shampoos and other personal care products. They serve as key ingredients that help to cleanse, emulsify, and create foam, removing dirt, impurities from the skin and hair. CAPB has been increasingly used in personal care formulations because of its foam boosting and high mildness properties. This study aimed to develop and validate method for the quantification of CAPB in shampoo using HPLC-RI detector. All validation parameters were conducted in compliance with the ICH guidelines and AOAC International Appendix K and were found to be within permissible limits. R² for linearity was greater than 0.99. LOD and LOQ were 60 µg/mL and 200 µg/mL respectively. The % RSD for method precision was 0.85%. The recovery range was found within 95–98%. The developed method is precise, robust, independent of the Shampoo matrix and thus suitable for routine for CAPB identification and quantification in Shampoo.