Evaluation of protein and lipid on feed of Tilapia Oreochromis niloticus

The objective of this research was to determine the optimum level of protein and lipid in improving the growth performance of Nile tilapia Oreochromis niloticus. This study was conducted using a 2 x 2 randomized factorial design with two treatment factors: the level of protein (28% and 32%) and the level of lipid (6% and 8%). So there were four test treatments and carried out with three replications. Nile tilapia with an average body weight of 5.60 ± 0.06 g were reared in the 900×45×35 cm aquarium at a density of 20 fish/aquarium. The experimental fish were reared for 60 days and fed three times daily to apparent satiation levels. Parameters evaluated in this study were growth performance and blood chemistry. The fish fed with diet 32;8 showed significantly higher growth than other treatments. Plasma protein in the fish fed with 32;6 diets were higher than those of plasma triglycerides, high density lipoprotein, and low density lipoprotein content were the same among the treatments. The result shows that feed 28;8 has the best protein efficiency ratio and protein retention. Keyword: Oreochromis niloticus, protein sparing effect, lipid, feed. ABSTRAK Tujuan penelitian ini adalah untuk mengetahui kadar protein dan lipid yang optimum dalam meningkatkan kinerja pertumbuhan ikan nila Oreochromis niloticus. Penelitian ini menggunakan rancangan acak faktorial 2 x 2 dengan dua faktor perlakuan yaitu kadar protein (28% dan 32%) dan kadar lipid (6% dan 8%). Jadi terdapat empat perlakuan uji dan dilakukan dengan tiga kali ulangan. Ikan nila dengan rata-rata bobot badan 5,60 ± 0,06 g dipelihara dalam akuarium berukuran 900×45×35 cm dengan kepadatan 20 ekor/akuarium. Ikan dipelihara selama 60 hari dan diberi makan tiga kali sehari secara at satiation. Parameter yang dievaluasi dalam penelitian ini adalah kinerja pertumbuhan dan kimia darah. Ikan yang diberi perlakuan dengan pakan 32;8 menunjukkan pertumbuhan yang lebih tinggi dibandingkan perlakuan lainnya. Protein plasma pada ikan yang diberi pakan 32;6 lebih tinggi dibandingkan dengan trigliserida plasma, HDL dan LDL rendah yang sama antar perlakuan. Hasil penelitian menunjukkan bahwa pakan 28;8 mempunyai nilai protein efesiensi rasio dan retensi protein terbaik.