Introduction to the Festschrift for Paul Walther

Journal of MicroscopyVolume 299, Issue 3 pp. 179-185 EDITORIAL Introduction to the Festschrift for Paul Walther Clarissa Read, Corresponding Author Clarissa Read [email protected] Central Facility for Electron Microscopy, Ulm University, Ulm, Germany Correspondence Clarissa Read, Central Facility for Electron Microscopy, Ulm University, Ulm, Germany. Email: [email protected]Search for more papers by this authorUlla Neumann, Ulla Neumann Central Microscopy, Max Planck Institute for Plant Breeding Research, Köln, GermanySearch for more papers by this author Clarissa Read, Corresponding Author Clarissa Read [email protected] Central Facility for Electron Microscopy, Ulm University, Ulm, Germany Correspondence Clarissa Read, Central Facility for Electron Microscopy, Ulm University, Ulm, Germany. Email: [email protected]Search for more papers by this authorUlla Neumann, Ulla Neumann Central Microscopy, Max Planck Institute for Plant Breeding Research, Köln, GermanySearch for more papers by this author First published: 11 August 2025 https://doi.org/10.1111/jmi.70023Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookxLinkedInRedditWechatBluesky REFERENCES 1Walther, P. (1984). Beiträge zur Verbesserung der Oberflächeninformation in der Rasterlelektronenmikroskopie biologischer Objekte. Dissertation, Doctoral Thesis. Naturwissenschaften ETH Zürich, Nr. 7647. Google Scholar 2Käppeli, O., Walther, P., Mueller, M., & Fiechter, A. (1984). Structure of the cell surface of the yeast Candida tropicalis and its relation to hydrocarbon transport. Archives of Microbiology, 138, 279–282. 10.1007/BF00410890 CAS PubMed Web of Science® Google Scholar 3Walther, P., Müller, M., & Schweingruber, M. E. (1984). The ultrastructure of the cell surface and plasma membrane of exponential and stationary phase cells of Schizzosaccharomyces pombe, grown in different media. Archives of Microbiology, 137, 128–134. 10.1007/BF00414453 CAS Google Scholar 4Walther, P., Kriz, S., Müller, M., Ariano, B. H., Brodbeck, U., Ott, P., & Schweingruber, M. E. (1984). Detection of protein A gold (15 nm) marked surface antigens by backscattered electrons. Scanning Electron Microscopy, 1984(Part 3), 1251–1266. Google Scholar 5Humbel, B., & Müller, M. (1986). Freeze substitution and low temperature embedding. In M. Müller, R. P. Becker, A. Boyde, & J. J. Wolosewick, (Eds.), The science of biological specimen preparation (pp. 175–183). SEM, AMF O'Hare. Google Scholar 6Autrata, R., Walther, P., Kriz, S., & Müller, M. (1986). A BSE scintillation detector in the (S)TEM. Scanning, 8, 3–8. 10.1002/sca.4950080103 CAS Web of Science® Google Scholar 7Walther, P., Schweingruber, A. M., Müller, M., & Schweingruber, M. E. (1988). Morphological organization of glycoprotein containing cell surface structures in yeast. Journal of Ultrastructure and Molecular Structure Research, 101, 123–136. 10.1016/0889-1605(88)90002-X CAS Web of Science® Google Scholar 8Moor, H. (1987). Theory and practice of high pressure freezing. In R. A. Steinbrecht, & K. Zierold (Eds), Cryotechniques in biological electron microscopy (pp. 175–191). Springer Verlag Berlin Heidelberg. 10.1007/978-3-642-72815-0_8 Google Scholar 9Studer, D., Michel, M., & Müller, M. (1989). High pressure freezing comes of age. Scanning Microscopy, Supplement, 3, 253–268. 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High-resolution cryo-SEM allows direct identification of F-actin at the inner nuclear membrane of Xenopus oocytes by virtue of its structural features. Journal of Microscopy, 232, 379–385. 10.1111/j.1365-2818.2008.02109.x CAS PubMed Web of Science® Google Scholar 22Walther, P. (2003). Recent progress in freeze fracturing of high-pressure frozen samples. Journal of Microscopy, 212, 34–43. 10.1046/j.1365-2818.2003.01236.x CAS PubMed Web of Science® Google Scholar 23Aoyama, K., Takagi, T., Hirase, A., & Miyazawa, A. (2008). STEM tomography for thick biological specimen. Ultramicroscopy, 109, 70–80. 10.1016/j.ultramic.2008.08.005 CAS PubMed Google Scholar 24Höhn, K., Sailer, M., Wang, L., Lorenz, M., Schneider, M. E., & Walther, P. (2011). Preparation of cryofixed cells for improved 3D ultrastructure with scanning transmission electron tomography. 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FIB/SEM tomography with TEM-like resolution for 3D imaging of high-pressure frozen cells. Histochemistry and Cell Biology, 138, 549–556.27. 10.1007/s00418-012-1020-6 CAS PubMed Web of Science® Google Scholar 28Villinger, C., Neusser, G., Kranz, C., Walther, P., & Mertens, T. (2015). 3D analysis of HCMV induced-nuclear membrane structures by FIB/SEM tomography: Insight into an unprecedented membrane morphology. Viruses, 7, 5686–5704. 10.3390/v7112900 CAS PubMed Google Scholar 29Laue, M. (2025). Diagnostic electron microscopy in human infectious diseases – Methods and applications. Journal of Microscopy, 299, 186–205. 10.1111/jmi.13370 Web of Science® Google Scholar 30Wieczorek, S., & Krijnse Locker, J. (2025). Scanning transmission electron tomography to study virus assembly: Review for the retirement of Paul Walther. Journal of Microscopy, 299, 206–211. 10.1111/jmi.13374 Web of Science® Google Scholar 31El Hankouri, M., Nousch, M., Poddar, A., Müller-Reichert, T., & Fabig, G. (2025). In situ quantification of ribosome numbers by electron tomography. Journal of Microscopy, 299, 212–227. 10.1111/jmi.13380 Google Scholar 32Heinz, V., Rachel, R., & Ziegler, C. (2025). Application of STEM tomography to investigate smooth ER morphology under stress conditions. Journal of Microscopy, 299, 228–241. 10.1111/jmi.70020 Google Scholar 33Wanner, G., Schroeder-Reiter, E., & Assaad, F F. (2025). Cryo-SEM and large volume FIB-SEM of Arabidopsis cotyledons: Degradation of lipid bodies, biogenesis of glyoxysomes and reorganization of organelles during germination. Journal of Microscopy, 299, 242–267. 10.1111/jmi.13381 Google Scholar 34Felder, E., Rüth, J L., Abu-Omar, B., Wohlwend, M., Walther, P., & Read, C. (2025). High-pressure freezing of mechanically stretched cells. Journal of Microscopy, 299, 268–276. 10.1111/jmi.13411 Web of Science® Google Scholar 35Neusser, G., Philipp, T., & Kranz, C. (2025). Visualization and differentiation of binder components in hard carbon composite anodes by osmium tetroxide and uranyl acetate staining. Journal of Microscopy, 299, 277–286. 10.1111/jmi.70014 Google Scholar 36Kniesel, H., Poonam, P., Payer, T., Bergner, T., Hermosilla, P., & Ropinski, T. (2025). DeepEM playground: Bringing deep learning to electron microscopy labs. Journal of Microscopy, 299, 287–300. 10.1111/jmi.70005 Web of Science® Google Scholar Volume299, Issue3Special Issue: Festschrift for Professor Paul WaltherSeptember 2025Pages 179-185 ReferencesRelatedInformation