This protocol outlines the culture and transfection of dopaminergic neurons from rat midbrain, highlighting key methods and applications.
Dopaminergic neurons consist some of the most difficult cells to culture due to their scarcity, intricate arborization and intrinsic vulnerability. The culture of primary VM neurons from murine brains has advanced the understanding of neuronal function at the cellular and molecular level. This protocol presents an established method to isolate and culture dopaminergic neurons from rat midbrain brains along with a method to sparsely transfect dopaminergic neurons.
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Pulido et al. (2025) studied this question.
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