This protocol details optimized deparaffinization for human brain tissue, facilitating optical clearing and 3D reconstruction using fluorescent labeling.
This protocol is a detailed version of the deparaffinization protocol for human brain tissue applicable to different clearing protocols (SHORT and iDISCO). The method was optimized by the European Laboratory for Non-Linear Spectroscopy - University of Florence -Italy, and describes the process to deparaffinize human brain tissue to prepare them for optical clearing and fluorescent labelling, permitting the 3D reconstruction of the tissue at micrometer-resolution using fluorescence microscopy techniques.
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Meo et al. (2025) studied this question.
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