QuPath Stain Quantification of Whole Tissue by Percent Area v1

This protocol utilizes QuPath, an open-source digital pathology software, to quantify histological stains (nuclear and non-nuclear(nuclear and non-nu) based on the percentage area of positive stain across whole tissue sections. It enables the assessment of non-nuclei stains, including commonly used markers such as TUNEL, to detect apoptosis, and Masson's Trichrome, to detect fibrosis and collagen deposition. This approach provides a reproducible protocol for whole tissue stain quantification.