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This is a In Vitro Study study (also classified as: Observational).

August 17, 2025

Modelling 16:8 Intermittent Fasting and Breakfast-Skipping in Mouse Adipocytes 3T3-L1 In Vitro: A Transcriptomics Study

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The 16:8 intermittent fasting regimen reduced lipid accumulation by 74.41% compared to control, promoting lipolysis.
Lipid accumulation was significantly reduced with a p-value of 0.007, while breakfast-skipping showed negligible impact with p=0.999.
Whole transcriptome sequencing highlighted significant gene modulation in adipogenesis and glycolysis pathways during fasting regimens.
The findings indicate that differentiated 3T3-L1 adipocytes serve as a valuable in vitro model for studying the effects of fasting protocols on lipid metabolism.

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Implication

Experimental study reveals 16:8 intermittent fasting significantly reduces lipid accumulation in 3T3-L1 adipocytes, suggesting its potential in obesity treatment.

Cite This Study

Er et al. (2025) studied this question.

synapsesocial.com/papers/68a36ddf0a429f797333126a https://doi.org/10.21203/rs.3.rs-7322953/v1

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Implication

Experimental study reveals 16:8 intermittent fasting significantly reduces lipid accumulation in 3T3-L1 adipocytes, suggesting its potential in obesity treatment.

Modelling 16:8 Intermittent Fasting and Breakfast-Skipping in Mouse Adipocytes 3T3-L1 In Vitro: A Transcriptomics Study

Key Points

Discussion

Implication

Cite This Study

Modelling 16:8 Intermittent Fasting and Breakfast-Skipping in Mouse Adipocytes 3T3-L1 In Vitro: A Transcriptomics Study

Key Points

Discussion

Implication

Cite This Study

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