Protein pattern and peroxidase activity in normal and tumor pumpkin tissues

Explanted pumpkin (Cucurbita pepo L.) cotyledons were susceptible to tumor transforma­tion. Crown gall tumors were induced using Agrobacterium tumefaciens (wild strain B6SJ/pTi). Tumor proliferation on intact plants was not achieved. Permanent crown gall tissue line was established on a MS medium without any growth regulators. Peroxidase activity in tumor and callus tissue lines was sig­nificantly higher than that in the plant organs. The electrophoretic protein patterns of tumor and dif­ferent callus tissue lines seemed to be similar, showing only slight differences between embryogenic and non-embryogenic callus lines, or between normal and tumor tissues.