Physicochemical Evaluation of Streptomycin Sulphate using Simple and Newly Developed Spectrophotometric Methods

Streptomycin sulphate (STP), an antibiotic that belongs to the aminoglycoside class, works by blocking the protein synthesis of the bacteria. This research presents a simple, fast, and cost-effective method for the analysis of the physicochemical parameters of STP used by three brands. The current study was conducted in two stages. In the first stage, physical properties such as color, pH, and weight variations of brands containing STP were evaluated using simple aesthetic procedures. In the second stage, chemical analysis was carried out using derivatization approach, followed by analysis with spectrophotometer. The derivatization of STP was carried out using vanillin as derivatizing agent. Beer’s law was followed in the range of 10-50 µg/ml, while calibration curve was obtained in the same range with R2 value of 0.9993, which shows the linearity of the method. The analysis was carried out using the recovery method. There was 99-101% recovery of the drug using the new method with relative standard deviation ranging between 0.19-0.22%. The results showed that the new method is accurate and precise.