Selective Isolation of Lipid Classes from cold-water corals via SPE for Quantitative Fatty Acid Profiling v1

This protocol describes the extraction of total lipids from coral polyp samples using the method of Bligh and Dyer (1959), followed by the separation of lipid classes via solid phase extraction (SPE) on aminopropyl (NH₂) silica cartridges as described by Kaluzny et al. (1985). A one-step acidic transesterification, adapted from Indarti et al. (2005) and Christie (2003), is then applied to produce fatty acid methyl esters (FAMEs) for GC-MS analysis.