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August 18, 2025Frontiers in Plant ScienceOpen Access

Molecular detection tool for prompt, reliable and precise diagnosis of the rice weevil, Sitophilus oryzae (Linnaeus) infestation in wheat

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Authors

KNK.C. NagpalPJPoonam JasrotiaMJMaha Singh Jaglan

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Overview

This molecular diagnostic assay detects Sitophilus oryzae in wheat quickly, indicating strong support for biosecurity measures.

Key Points

  • The assay achieves reliable detection of S. oryzae in stored wheat grains, ensuring rapid pest management actions.
  • With a sensitivity threshold of just 1 picogram of DNA, the assay can detect a single pest in 10 kg of grains.
  • Utilizing conventional and quantitative real-time PCR methods, the assay demonstrates high specificity with no cross-reactivity.
  • Its implementation in grain storage facilities can enhance pest surveillance and biosecurity protocols effectively.

Cite This Study

Nagpal et al. (2025) studied this question.

synapsesocial.com/papers/68af453aad7bf08b1ead277fhttps://doi.org/10.3389/fpls.2025.1628692
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