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August 19, 2025Behavior Research MethodsOpen Access

Reliability and reactivity of heart rate variability and pupillometry in response to controlled autonomic perturbations in university students

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Test-retest reliability study shows HRV and pupillometry respond to cognitive testing and breathing in students, suggesting psychological effects.

HRV and pupillometry reliably detect physiological changes in response to cognitive and respiratory stimuli.
Strong day-to-day reliability confirmed with ICC values ranging from 0.66 to 0.87 for both metrics under varied conditions.
The virtual reality protocol effectively induced measurable changes in heart rate variability and pupillometry metrics across sessions.
While detecting individual changes may be challenging, group comparisons remain valid due to strong reliability of the metrics.

Matyevich et al. (2025) studied this question.

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Test-retest reliability study shows HRV and pupillometry respond to cognitive testing and breathing in students, suggesting psychological effects.