Enhancements in transformation frequency for bacillus subtilis with optimized Mn2+ medium, indicating greater efficiency.
To enhance the transformation efficiency of Bacillus subtilis Marburg 168 using PCR DNA fragments, we optimized the competent cell preparation by adding Mn2+ to the competence medium. This Mn2+ addition promoted both cell growth and increased transformation frequency. Moreover, we determined the necessary length of the homologous sequence on each end to achieve practically sufficient transformation through double crossover recombination.
No takes yet. Share an insight, caveat, or question.
Sawada et al. (2025) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: