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August 17, 2025Open Access

Mitochondrial isolation from cultured fibroblasts v1

Ashley W. Seifert Vekaria Hemendra Ebenezer Aryee

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Key Points

Mitochondrial isolation is achieved using a detailed procedure for fibroblasts, ensuring purity and functionality of organelles.
Key parameters include specific techniques followed in the Seifert Lab protocol to enhance mitochondrial yield and quality.
The approach involves a series of centrifugation steps that separate mitochondria from cytoplasmic components in cultured cells.
This method potentially supports further studies on mitochondrial function and bioenergetics in fibroblast cells.

Implication

This protocol outlines mitochondrial isolation methods from cultured fibroblasts, indicating application in cell biology.

Abstract

This protocol describes the procedure used by the Seifert Lab to isolate mitochondria from cultured fibroblasts.

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Cite This Study

Seifert et al. (2025) studied this question.

synapsesocial.com/papers/68af4cdfad7bf08b1ead666c https://doi.org/10.17504/protocols.io.81wgbwxr1gpk/v1

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