Irisin‐Loaded Dissolving Microneedles Promote Rapid Wound Healing

Irisin, an exercise‐induced myokine with anti‐inflammatory and regenerative activity, is incorporated into dissolving poly(vinyl alcohol)/sucrose microneedle (MN) patches to enhance cutaneous wound repair. Recombinant irisin (0.5–1 μg mL −1 ) is noncytotoxic to human fibroblasts and keratinocytes and significantly accelerates their migration in scratch and coculture assays. Dual‐layer MNs exhibit sharp geometry and adequate fracture strength (0.08 N > 0.058 N insertion threshold) and release >90% of the loaded irisin within minutes. In a rat dorsal‐wound model, both topical irisin and irisin‐MN treatment hasten closure, but MN delivery produces deeper penetration and greater bioavailability, upregulating collagen III, SNAP25, and TGF‐ β 1 while limiting excessive inflammation on H&E sections. Histology confirms thinner, better‐organized granulation tissue and more complete reepithelialization in the irisin‐MN group. These findings demonstrate that dissolving MNs provide a minimally invasive platform for localized irisin delivery, coupling the myokine's promigratory, angiogenic, and anti‐inflammatory actions with efficient transdermal transport to achieve rapid, high‐quality wound healing.