The effects of tobacco straw on the rhizosphere soil microbial community structure of bobacco (healthy tobacco and black shank tobacco)

As a vital agricultural management strategy, straw returning enhances soil fertility and promotes crop growth. Tobacco straw shows promise as an organic amendment for soil improvement; however, its impact on soil microbial diversity and community structure remains unclear. This investigation focused on the effects of tobacco straw on the rhizosphere soil microbial community structure of healthy tobacco and black shank tobacco, utilizing high-throughput sequencing technology. The results indicated that the abundance and diversity of microbial communities decreased following the addition of tobacco straw. In terms of community structure, the abundance of species such as Proteobacteria, Bacteroidetes, Flavobacterium, and Pseudomonas increased after the addition of tobacco straw, potentially inhibiting the onset of black shank disease. Conversely, the abundances of Actinobacteria, Ascomycota, and Fusarium decreased following straw addition. Random forest model analysis revealed that the most representative genera in the healthy tobacco rhizosphere soil were Acidibacter and Luteimonas, while Herbinix served as the corresponding biomarker in the black shank tobacco rhizosphere soil.