Figure S3 from Reactivating cGAS–STING Signaling by Targeting SOS1 Enhances Antitumor Immunity in NRAS-Mutant Tumors

Supplementary Fig. S3. The suppression of cGAS-STING pathway in NRAS mutant cells mediates immune escape. A. Flow cytometry measuring the DCs mature markers (MHC II and CD86), when co-cultured with the indicated tumor cells pre-treated with poly(dA:dT). B. Immunoblot in NCI-H1299 cells expressing sgRNA targeting STING. C. Flow cytometry measuring the DCs mature markers (MHC II and CD86), when co-cultured with the indicated tumor cells pre-treated with HT-DNA. D-E. Flow cytometry measuring the cytotoxicity of PBMCs that killing the cancer cells pre-treated with poly(dA:dT) (D) and HT-DNA(E). F. FACS analysis of CD8⁺T, NK1.1⁺, and IFNγ⁺ or Granzyme B⁺ in CD8⁺ T cell populations and NK cell populations from the isolated TILs. Representative dot plots from a representative mouse for each group.