This research demonstrates a refined extraction method for high-molecular-weight DNA in Zymoseptoria tritici, indicating improved sequencing outcomes on multiple platforms.
Long-read sequencing is increasingly accessible, enabling unprecedented insights into the pervasive structural rearrangements of fungal genomes. In this study, we optimized a genomic DNA extraction protocol to obtain unsheared, high-molecular-weight DNA suitable for both PacBio and Oxford Nanopore Technologies sequencing. We validated the protocol with both sequencing platforms, achieving in both case telomere-to-telomere genome assembly of natural isolates of the fungal ascomycete Zymoseptoria tritici.
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Prieur et al. (2025) studied this question.
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